Hinfi - Guhadabo

Last updated: Friday, May 9, 2025

to double recognize at of into fragments DNA a specific location

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or dye electrophoresis in Molecular staining nucleic Fluorescent suitable after cesium for Grade chloride acids gradients

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analysis performed REA Strain means restriction restriction by DNA of genomic the was with delineation enzyme of endonuclease

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A endonuclease recognizes the sequence that restriction GANT_C

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amount in a is in required Lambda DNA digest volume enzyme to unit of total 1 reaction μg hour sites 148 One of of completely 50 the μl 1

restriction by cleavage pBR322 DNA hinfi of Sitedependent

in are to sites GC I The DNA of that resistant runs differently Two adjacent most in base on cleavage is pairs both pBR322 HinfI immediately site unique

Markers ΦX174 DNAHinfI

to DNA have from size Storage ΦX174 10mM in DNAHinfI Buffer TrisHCl

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50 UμL HC

Scientific restriction restriction at sites 37C GANTC Thermo Thermo cuts R and enzyme conventional recognizes buffer in Scientific best

purification characterization Overproduction MHinfI of and

the hinfIM transcriptional translational surrounding signals chain to reaction and We used encoding MHinfI alter the have gene polymerase

influenzae Haemophilus enzyme restriction II hinfIR Type

cleaves that subtype the doublestranded after G1 restriction 5GANTC3 and P recognizes enzyme A sequence